Ctcf antibody chip
WebChIP-sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade – BSA and Azide free (ab240035) Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab128873. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 … WebChromatin Immunoprecipitation - CTCF Rabbit pAb (A1133) Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody (A1133) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Ctcf antibody chip
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Web11 rows · Applications. Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC … WebMonoclonal Antibody for studying CTCF. Cited in 110 publications. Validated for WB, IP, IHC, IF, IF, ChIP, ChIP, C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, CTCF (D31H2) XP® …
WebDec 11, 2015 · Chromatin was obtained and subjected to fragmentation with an average length of 300 bp by sonication. The anti-PolII monoclonal antibody 8WG16 (Covance, MMS-126R) and anti-CTCF antibody (Abcam, ab70303) were used to enrich PolII and CTCF-bound chromatin fragments, respectively. ChIP DNA on beads was used for ChIA … WebCTCF Rabbit pAb (A12562) Western blot analysis of extracts of various cell lines, using CTCF antibody (A12562) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit …
WebAnti-CTCF (rabbit antiserum) Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, … WebThe ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG), and qPCR primers which amplify a 165 bp region of human H19 internal control region. The CTCF and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CTCF ...
WebJan 14, 2024 · ChIC/CUT&RUN sequencing for anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408). Figure 2. ChIC/CUT&RUN sequencing for anti-H3K4me3 antibody [EPR20551-225] - ChIP Grade (ab213224). Browse all our ChIC/CUT&RUN-seq validated recombinant antibodies. To learn more about our ChIC/CUT&RUN-seq validated …
WebFor ChIP-seq, chromatin was extracted and immunoprecipitation with antibodies was performed. All IPs in one biologocal replicate samples were processed together. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina kit and using NEBNext Multiplex Oligos. Libraries were amplified with 6-8 cycles of PCR. small timber framed cabinsWebThe ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG), and qPCR primers which amplify a 165 bp region of human H19 internal … highway to hell film wikipediaWebUse Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor. An … small timber retaining wallWebJan 7, 2024 · b CTCF ChIP-seq data for replicate LNCaP RNAi experiments and wildtype GM12878, K562, ... Antibody/protein complexes were bound to Salmon Sperm … small timber picnic tableWebAll lanes : Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) at 1/1000 dilution (un-purified) Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lysates/proteins at 10 µg … highway to hell gas cylinderWebUse Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor. An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells. highway to hell gitarre lernenWebJan 14, 2024 · As a complement to our genome-wide comparisons of CTCF and cohesin binding, we performed a thorough analysis of CTCF antibody performance in ChIP-seq, finding that a number of antibodies fail to detect the full spectrum of CTCF sites (SI Appendix, Fig. S3). This observation suggests that the high prevalence of CNC sites in … small timber lodge index wa