Fitc mouse anti-human cd31
WebMonoclonal Anti-CD31-FITC antibody produced in mouse. clone MEM-05, purified immunoglobulin, buffered aqueous solution. View Price and Availability. Sigma-Aldrich. 131M-9. ... Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in … WebJan 21, 2024 · Conjugate/Tag/Label : FITC. Application Notes : Clone WM59 has been reported to recognize the D2 extracellular portion of CD31. Additional reported …
Fitc mouse anti-human cd31
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WebOct 5, 2024 · Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31. 8 Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo blocking of CD31-mediated cell-cell interactions 1-4 , and immunohistochemical staining 5,6,7 of acetone-fixed frozen …
WebValidated in WB, IHC, Flow Cyt and tested in Mouse, Rat, Human. Cited in 282 publication(s). Independently reviewed in 57 review(s). ... Anti-CD31 antibody [P2B1 ... Samples were incubated with primary antibody (1/10) overnight at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (1/200) was used as the secondary antibody. Flow … WebBlue pseudocolor = DRAQ5 ™ (fluorescent DNA dye). Western blot analysis of extracts from HUVEC cells using CD31 (PECAM-1) (89C2) Mouse mAb. Immunoprecipitation of CD31 from HUVEC cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is CD31 (PECAM-1) (89C2) Mouse mAb.
WebCD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an ... WebApr 7, 2024 · FITC-dextran permeability assay ... monoclonal antibody Alexa Fluor® 555 (Cell Signalling Technology) mouse monoclonal human CD31 Alexa Fluor 488-, 594- and 647-conjugated antibodies (BioLegend ...
WebThe CD31 (Anti-PECAM-1) antibody, clone L133.1, is derived from the fusion of Sp2/0 mouse cells with spleen cells from a BALB/c mouse immunized with purified human …
WebMar 12, 2024 · Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD105 ( MCA1557A647 ) and FITC conjugated Mouse anti Human CD31 (MCA1738F). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. product information. dungeness fish shackWebWhole blood was stained with either Purified Mouse Anti-Human CD31 (Cat. No. 555444/550389; solid line histograms) or Purified Mouse IgG1, κ Isotype Control (Cat. … dungeness high tide timesWebCD31/PECAM‑1 in HUVEC Human Cells. CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody … dungeness hatchery sequim washingtonWebImmunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100. FC experiment of BALB/c mouse … dungeness historic district cumberland islandWebAPC anti-mouse CD31 Antibody - CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. ... Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. 8999 BioLegend Way, ... FITC anti … dungeness instream flow ruleWebC57BL/6 mouse splenocytes were stained with anti-mouse CD3 APC/Cyanine7 and anti-mouse CD31 (clone W18222B) FITC (left) or rat IgG2b, κ isotype control (right) ... Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. 8999 BioLegend Way, San ... dungeness golf courseWebJan 8, 2024 · The following six parameters were used to discriminate cells in the experiments: Hoechst 33342 (red), Hoechst 33342 (blue), forward scatter, side scatter, PerCP-Cy5.5-CD45, FITC-SCA1 and PE-CD31 reactivity (green) as previously described (14,27,30). This protocol is illustrated in Fig. 1. The Hoechst dye was excited at 350 nm. dungeness irrigation group