Hct116 p53-/-

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August undefined, 2024

WebHCT116 p53–/– cells as well as a derivative cell line expressing the p53-Luc fusion were tested and the data presented are an average of three independent experiments. Activation of the p53 protein protects the organism against the propagation of cells that carry damaged DNA with potentially transforming mutations. MDM2, a p53-specific E3 ... WebApr 7, 2024 · In line with these, we observed protein levels of DDX21 in most of CRC cell lines, such as HCT116, LOVO and RKO, ... According to our GSEA analysis result, P53 …

Regulation of p53 stability and function in HCT116 colon

WebJan 5, 2024 · The p53-null HCT116 p53 (-/-) cells were transduced HCT116 stable expressing p53 R273H and p53 R248W cell lines and were generated by transduction with pCMV-Neo-Bam mutp53 plasmids expressing mutant p53 with R273H or R248W into HCT116 p53 (-/-) cells (pCMV-Neo-Bam-p53 R273H and R248 mutation vectors were a … WebJul 20, 2024 · Comparable radiosensitivities of p53 +/+ and p53-/-HCT116 colorectal cells induced by CIR were demonstrated, as well as persistent 53BP1 foci indicated DNA … fitx hamburg steilshoop

Phase separation of DDX21 promotes colorectal cancer metastasis …

WebFeb 27, 2004 · Regulation of p53 stability and function in HCT116 colon cancer cells We have used a lentiviral vector to stably express p53 at a physiological level in p53 … WebP53 and MDM2 co-occupy enhancers and promoters associated with P53 pathways. When highly expressed, MDM2 also binds an additional set of P53-independent promoters that engage in multi-way physical interactions and regulate genes involved in cell division, RNA splicing, translation and stress responses. WebTherefore, HCT116 p53+/+ cells are simply marked as HCT116 except for in Figure 1. Our results confirmed that ES induced apoptosis by reducing the expression of c-Myc and … can i grow cherries in florida

Arrested development: When cells make mistakes

WebTherefore, HCT116 p53+/+ cells are simply marked as HCT116 except for in Figure 1. Our results confirmed that ES induced apoptosis by reducing the expression of c-Myc and checking cell viability and protein levels in HCT116 and HT29 cells. ES also regulates the expression levels of c-Myc, pro-PARP, cleaved-PARP, pro-Caspase 3, and cleaved ... WebHCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for … fitx hamburg coronaWebTo establish p53 dependence more clearly, we compared protein levels from HCT116 wt and HCT116 p53 −/− cells, following D4476 treatment (Fig. 5, B and C). In HCT116 wt cells ( Fig. 5 B ), the p53, MDM2, p21, and E2F-1 protein levels were affected in the same manner as in A375 cells after D4476 treatment ( Fig. 2 A ), which indicated that ... fitx for fit

"WebATR and ETV1 promote TERT expression in human cancer cell lines expressing a p53 mutant and in HeLa cells. (A) Immunoblot analysis monitoring TERT levels in various … " - Hct116 p53-/-

Hct116 p53-/-

Phase separation of DDX21 promotes colorectal cancer metastasis …

WebAug 8, 2014 · We verified by PCR however, the HCT116 p53-/- still express all the p53 isoform (D40p53alpha,beta,gamma), d133p53alpha,beta,gamma and also the … WebJun 21, 2024 · We recently used siRNA to knock down a gene in the HCT116 cell line. The knockdown had no effect on target gene expression, whereas overexpression had a minor effect in the same cell line and...

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WebJan 12, 2024 · Wild-type HCT116 cells (HCT116/p53 +/+) and p53 knockout HCT116 cells (HCT116/p53 −/−) were a gift from Dr. Yao Lin (Fujian Normal University, Fujian, China) … WebWe were able to confirm that the colon cancer cell lines LIM1215 and HCT116, as well as the breast cancer cell line MCF7, have wild-type p53, while the colon cancer cell line Caco-2, as well as...

HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. Furthermore, it was found that the knockout of MARCH2 limited …

WebLanguage Label Description Also known as; English: HCT 116 TP53(-/-) cell line WebJan 15, 2024 · HCT116 p53-isogenic cells were obtained from B. Vogelstein (Johns Hopkins University, Baltimore, MD) and HCT116 p21-isogenic cells from M. Dobbelstein (Georg-August University, Göttingen, Germany). RKO p53-isogenic lines were a gift from K. Vousden (Beatson Institute, Glasgow, United Kingdom).

WebJan 5, 2024 · Two HCT116 cell lines containing hot spots at codons R273H and R248W of p53 were constructed based on HCT116 with knockout p53, respectively. Cell viability, …

WebJan 2, 2024 · Preferentially upregulated proteins were enriched with members of the steroid biosynthesis, cytokine–cytokine receptor interaction, mitophagy and ferroptosis pathways, suggesting that HCT116... fitx helpWebApr 16, 2024 · USP11 was introduced into A549 (p53 +/+) as well as two HCT116 cell lines with a p53 WT (HCT116 WT) and null (HCT116 p53 −/−) genotype. Interestingly, USP11 overexpression resulted in a significant increase of endogenous p21 levels ( Fig. 2 A ), and increasing USP11 expression caused an elevation of p21 levels in a dose-dependent … fitx hesslerWebApr 7, 2024 · In line with these, we observed protein levels of DDX21 in most of CRC cell lines, such as HCT116, LOVO and RKO, ... According to our GSEA analysis result, P53 pathway, KRAS signaling pathway ... can i grow chives in waterWebThe P53 mutation stimulated hyper-proliferation and chemo-resistance of tumor cells and enhanced invasion/metastasis . The anti-cancer efficacy of oxaliplatin, 5-FU, cisplatin depended on the p53 status in tumor cells [44,45]. The GI 50 value of the MI-1 for human colon carcinoma HCT116 p53-/- cells with the deletion of P53 gene equaled 0.89 ... fitx halleWebWild-type and p53 knockout HCT116 cell lysates were kindly provided by a collaborator. Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [PAb 240] (ab26) ab26 stained in A431 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA … fitx holthausenWebMay 1, 2007 · Drug treatment of HCT116 cells triggers immediate stabilization of p53 protein (as evidenced by Western blots) and subsequent suppression or activation of a great number of p53-controlled genes involved in DNA damage response (e.g. CHK1, RAD54, H2AFX), DNA replication arrest (e.g. CDC6, CDC7, RRM2, MCM 2-7, CDT1, RFC3, … fitx hamburg flughafenWebHCT116 knockout cells were infected with a blank or a p53-expressing lentivirus; HCT116 parental p53 wt cells were infected with a blank … fitx hilfe