Hct116 puromycin
WebHCT 116 Cell Line. HCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into … WebOct 28, 2013 · HCT116 TCF4/Wnt cells were cultured in the presence of 50 μg ml −1 hygromycin B, and HCT116 TCF/LEF cells were cultured in the presence of 10 μg ml −1 puromycin. Primary colon cancer ...
Hct116 puromycin
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WebThis Luciferase & GFP / human HCT116 cell line was transformed via Lentivirus transduction. It has the Luciferase and GFP was constitutively co-expressed under EF1a promoter, as two separate proteins (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following … WebHCT116-Fluc-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental HCT116 cells …
WebPuromycin titration in HCT116 cells. Cells were treated with given concentrations of puromycin. Cell death was microscopically monitored over time. Scale bar: 50 µm. 4 x … WebGeneticin (G418 sulfate), zeocin, hygromycin B, puromycin, and blasticidin antibiotics are the most common selection antibiotics used for stable cell transfection. These antibiotics provide unique solutions for your research needs, such as dual selection and rapid, stable cell line establishment. Explore all Gibco selection antibiotics
WebDec 18, 2024 · Antibiotics selection with puromycin and blasticidin S increased the efficiency to knock cassettes with selection markers into the target genomic regions (38.7% for 5′, 34.4% for 3′ blasticidin S... WebDec 30, 2024 · Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose product, puromycin acetyltransferase, inactivates puromycin via acetylation; recommended concentration in cell culture 1-10µg/ml. Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of …
WebNegative control and ZCRB1-knockdown HCT116 cells were cultured in DMEM (GIBCO, Thermo Scientific) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin solution (Gibco), 1μg/mL puromycin (Invivogen) and 3 μM Doxycycline (Selleck) at 37°C under 5% CO2. Extracted molecule:
WebApr 1, 2024 · Stable transfection of HCT116 p53 +/+ and HCT116 p53 −/− cell lines (a kind gift from Dr. B. Vogelstein) were performed as described previously [17], Transfected cells were selected by culturing in medium containing 4 μg/ml of puromycin (Sigma-Aldrich), and single cell colonies were isolated. Immunoblots lagu mayang adik vanessa angelWebApr 12, 2024 · Puromycin was added to the cells for 5 days and individual cell colonies were isolated. The knockout effect was examined by Western blot with DNMT1 antibody (clone EPR3521(2); Abcam) in the ... jeep\\u0027s lqWebThis cell line stably expresses luciferase at high level. It also expresses a green fluorescent protein (GFP) and is resistant to puromycin. This cell line will be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments. Cell Line: HCT116. Reporters: Luciferase, GFP. Selection Marker: Puromycin lagu mayangsari harus malam iniWebJan 10, 2024 · Following puromycin selection we identified single clones of DLD-1 and HCT116 cells displaying a green fluorescence, which were isolated and expanded as monoclonal cell lines (Figure 6A). To confirm correct CB integration at the AAVS1 locus we performed PCR-based genotyping as illustrated in Figure 6 B (strategy outlined in Figure … lagu mazmur tanggapanWebJan 10, 2024 · Briefly, growth media containing DMEM (high glucose, pyruvate, ThermoFisher Scientific, Waltham, MA, USA) for HeLa Kyoto and HCT116 cells and … lagum biografiaWebHCT116 cells have been widely used in colorectal cancer research. HCT116/GFP (CMV) stable cells express GFP under the control of the CMV promoter. ... Puromycin: Biosafety Level: BSL-2: Shipped: Dry ice : Storage: Liquid Nitrogen: Catalog Number Product Size Price Qty; SC-1001. HCT 116/GFP (CMV) stable cells. 2x10^6 cells: Price: Quantity. … lagu mbah surip full albumWeb嘌呤霉素（Puromycin）是一种由白黑链霉菌产生的氨基苷类抗生素。其可以打乱核糖体上的肽转运，造成翻译过程中不成熟链终止，从而抑制蛋白质合成。在原核和真核细胞 … jeep\\u0027s ls