Human dutpase
WebOct 7, 2016 · In addition, a region with homologies to dUTPase proteins was found unexpectedly downstream from the integrase domain. Amino acid sequence and phylogenetic analysis indicate that the HERV-L pol gene is related to that of foamy retroviruses. ... Haynes RH. Human dUTP pyrophosphatase: cDNA sequence and … WebA recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 70-252 of Human dUTPase. Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI …
Human dutpase
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WebHuman Anatomy & Physiology. ... Through the action of dUTPase and its substrate specificity Despite similarity, dUTPase excludes thymine’s 5’-methyl group as well as similarly shaped cytosine as its substrates Only take up dUTP as substrate and hydrolyse it to dUMP, which can not be taken up as substrate for DNA synthesis any longer. WebWe previously performed a computational sequence analysis of the 5′ region of the human dUTPase gene and identified putative binding sites for NFκ-B, Sp1 and E2F (Supplementary Figure 1A). The presence of Sp1- and E2F-binding sites is consistent with the hypothesis that the dUTPase promoter is regulated in a cell-cycle-dependent manner ( 31 ).
WebJan 28, 2024 · The human herpesviruses dUTPase proteins act as Pathogen-Associated Molecular Pattern (PAMP) molecules and, through their ligation with Toll-like receptor (TLR)2 homodimers in the case of the EBV dUTPase or TLR2/1 heterodimers in the case of the HSV-2, HHV-6, HHV-8 and VZV dUTPases, differentially activate NF-κB, with … WebAug 1, 1997 · Expression of dUTPase isoforms during serum stimulation and serum starvation of 34Lu human lung fibroblasts. A, 34Lu cells were driven into G 0 by serum starvation for 72 h.
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WebThe nuclear isoform of human dUTPase, an enzyme that is essential for genomic integrity, has been shown to be phosphorylated on a serine …
Proteins in this study were expressed in E. coli BL21 (DE3) Rosetta cells with 0.5 l LB medium and being induced by 0.5 mM isopropyl β-D-1-thiogalactopyranoside IPTG at OD600 = 0.6. Stl (orf 20 of SaPIbov1, GI: 11094374) was expressed from a pGEX-4T-1 vector42 (see Supporting information), for 4 h at 30 °C. The … See more Native polyacrylamide gel electrophoresis was performed using 8% polyacrylamide gels as described previously42. Briefly, after 1 h of pre-electrophoresis at 100 V, 25 μl of pre-mixed samples were loaded on the gel and … See more ITC experiments were carried out at 20 °C on a Microcal ITC200 instrument (Malvern Instruments, Malvern, UK). Prior to the measurements hDUT and Stl samples were dialyzed overnight at 4 °C in a buffer of 20 mM HEPES … See more Proton release during the hydrolysis of dUTP into dUMP and PPi was followed continuously at 559 nm at 20 °C using a Specord 200 spectrophotometer (Analytic Jena, Germany) and 10 mm path length thermostatted … See more The protein complexes were studied using an unmodified commercial Waters QTOF Premier mass spectrometer (Waters, Milford, MA, USA) equipped with electrospray … See more goldfish socialWebSep 1, 1992 · The human dUTPase was found to contain five characteristics amino acid sequence motifs that are common to the dUTPases of E. coli, yeast, and herpesviruses and to dUTPase-like sequences encoded by some retrovirus gag and pol genes. A high degree of amino acid sequence identity (greater than 60%) was also observed between the … headache specialist ctWebdUTPase was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 μg ab229122. Western blot was performed from the immunoprecipitate using ab229122. Anti-Rabbit IgG was used as a secondary reagent. Lane 1: Control IgG IP in A431 whole cell extract. Lane 2: ab229122 IP in A431 whole cell extract. goldfish snack websiteWebA fragment encoding human dUTPase (GenBank accession no. U62891) was amplified from reverse-transcribed placental RNA (cDNA),purified,andclonedintothepCAL-n-EKvector.Positives were analyzed by DNA sequencing. Human dUTPase (CBP-fusion protein) was purified and assayed for dUTPase activity (see below). HPLC Analysis of … headache specialist dcWebNov 7, 2024 · dUTPase/DUT Protein, Human - MedChemExpress. 下一篇. HPD/HPPDase Protein, Human (His) - MedChemExpress. goldfish soap in a bagWebAug 7, 2003 · A high cellular dTTP: dUTP ratio is essential to avoid uracil incorporation into DNA, which would lead to strand breaks and cell death. We report the first detailed atomic-resolution structure of a eukaryotic dUTPase, human dUTPase, and complexes with the uracil-containing deoxyribonucleotides, dUMP, dUDP and dUTP. headache specialist dallasgoldfish social inc