WebIL-1β levels by Flow Cytometry When THP-1 cells were stimulated with 100 ng/mL E. coli LPS, the percentage of IL-1β was found to be 36.7%, and this value decreased to 1.7 after … WebJan 18, 2024 · 不同浓度OMVs作用THP-1细胞时,NLRP3和caspase-1的mRNA表达量较对照组显著升高(P0.05)。作用3 h时,50 μg/mL组NLRP3的mRNA表达量显著高于5 μg/mL …
鲍曼不动杆菌外膜囊泡诱发人单核-巨噬细胞THP-1的炎性反应
WebTHP1-def cells are derived from THP-1 human monocytic cells. These are the most commonly used model cell line for the study of inflammasome activation as they express … WebApr 14, 2024 · Escartín-Gutiérrez JR, Ponce-Figueroa M, Torres-Vega MÁ, Aguilar-Faisal L, Figueroa-Arredondo P. Transcriptional Activation of a Pro-Inflammatory Response (NF-κB, … comfort tech serene foam contour pillow
EV-A71 induced IL-1β production in THP-1 macrophages is …
To further investigate whether RIG-I can affect EV-A71-induced IL-1β release in an NLRP3-independent manner, NLRP3 KD THP-1 macrophages were transfected with siRIG-I and then infected with EV-A71. The expression levels of RIG-I, NLRP3, and EV-A71 VP0 were confirmed by Western blotting (Fig. 3A). The … See more TLRs are known to be implicated in pathogen-induced IL-1β production31. Among these TLRs, evidence suggests that TLR3 and TLR8 play roles in RNA virus … See more TLR engagement has been demonstrated to be sufficient to promote inflammasome activation in human monocytes. To test the effects of TLR3 and TLR8 … See more To determine the potential of viral RNAs in triggering IL-1β release, different amounts of EV-A71 vRNA were transfected into THP-1 macrophages. The expression … See more WebMar 27, 2024 · On the one hand, the activation of Nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome requires nuclear factor kappa B (NF-κB)-dependent … WebApr 13, 2024 · Mito-TEMPO and MCC950 increased caspase-1p20 and IL-1β expression in THP-1 macrophages, indicating that mitochondrial ROS and autophagy could also … comfort tech service now del rio